choline oxidase from alcaligenes sp.
CHOLINE OXIDASE
CAS: 9028-67-5
Molecular Formula:
choline oxidase from alcaligenes sp. - Names and Identifiers
choline oxidase from alcaligenes sp. - Physico-chemical Properties
| Appearance | powder |
| Color | yellow |
| Storage Condition | -20°C |
| MDL | MFCD00130787 |
choline oxidase from alcaligenes sp. - Risk and Safety
| Safety Description | S22 - Do not breathe dust. S24/25 - Avoid contact with skin and eyes. |
| WGK Germany | 3 |
| FLUKA BRAND F CODES | 10-21 |
choline oxidase from alcaligenes sp. - Reference
| Reference Show more | Note: part of our products can only provide part of the information, our company does not guarantee the authority of the information provided, only for the customer reference Exchange research. storage conditions: -20 °c Appearance: yellow ish orphan powder, one unit causes the formation of one micromole of hydroperoxide (half a micromole of quinoneine dye). Thermal stability : below 37 ℃ (pH 7.5, 10min) pH Stability : pH 7.0~9.0 (30 ℃, 2 hr) optimal temperature : 40~45 ℃ optimal pH : 8.0-8.5 Inhibitors : p-Chloromercuribenzoate, Cu ion, Co ion, Hg ion, Ag ion Structure : One mol of FAD is covalently bound to mol of the enzyme8) Isoelectric point : 4.1±0.1 Stabilizers : EDTA, bovine serumalbumin, amino acids (glycine, sodium glutate, etc.) |
choline oxidase from alcaligenes sp. - Introduction
CHOLINE OXIDASE(Choline Oxidase) is an enzyme found in certain bacteria and fungi that catalyzes the oxidation of CHOLINE. With a molecular weight of about 100,000 daltons, CHOLINE OXIDASE is an acid protease.
The main use of CHOLINE OXIDASE is to determine the content of CHOLINE in medical and biochemical analysis. Choline is an important neurotransmitter, and the measurement of choline levels in blood and tissues is important for the diagnosis of some diseases such as muscle weakness and Alzheimer's disease. CHOLINE OXIDASE can convert CHOLINE into carbon dioxide, formaldehyde and hydroperoxide. The enzyme is able to measure the amount of choline by detecting the formaldehyde or nitrogen dioxide produced by the reaction.
The preparation method of CHOLINE OXIDASE is mainly based on purification technology and recombinant DNA technology. There are various methods for obtaining CHOLINE OXIDASE from bacteria or fungi, including centrifugation, membrane separation, methanol precipitation, ion exchange chromatography, Gel chromatography and column chromatography.
Some safety information needs to be paid attention to when dealing with CHOLINE oxidation. CHOLINE OXIDASE has a certain degree of sensitization, so appropriate personal protective measures need to be taken during the operation, such as wearing laboratory gloves and eye protection. In addition, the storage and transportation of CHOLINE OXIDASE needs to be carried out at low temperatures, avoiding contact with oxygen and moisture to maintain its activity and stability. In the use of CHOLINE OXIDASE, should pay attention to comply with the relevant laboratory safety procedures, to avoid direct contact and inhalation of CHOLINE OXIDASE.
The main use of CHOLINE OXIDASE is to determine the content of CHOLINE in medical and biochemical analysis. Choline is an important neurotransmitter, and the measurement of choline levels in blood and tissues is important for the diagnosis of some diseases such as muscle weakness and Alzheimer's disease. CHOLINE OXIDASE can convert CHOLINE into carbon dioxide, formaldehyde and hydroperoxide. The enzyme is able to measure the amount of choline by detecting the formaldehyde or nitrogen dioxide produced by the reaction.
The preparation method of CHOLINE OXIDASE is mainly based on purification technology and recombinant DNA technology. There are various methods for obtaining CHOLINE OXIDASE from bacteria or fungi, including centrifugation, membrane separation, methanol precipitation, ion exchange chromatography, Gel chromatography and column chromatography.
Some safety information needs to be paid attention to when dealing with CHOLINE oxidation. CHOLINE OXIDASE has a certain degree of sensitization, so appropriate personal protective measures need to be taken during the operation, such as wearing laboratory gloves and eye protection. In addition, the storage and transportation of CHOLINE OXIDASE needs to be carried out at low temperatures, avoiding contact with oxygen and moisture to maintain its activity and stability. In the use of CHOLINE OXIDASE, should pay attention to comply with the relevant laboratory safety procedures, to avoid direct contact and inhalation of CHOLINE OXIDASE.
Last Update：2024-04-09 20:52:54
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Featured ProductsMultiple SpecificationsSpot supply
Product Name: Choline oxidase Visit Supplier Webpage Request for quotationCAS: 9028-67-5
Tel: 18301782025
Email: 3008007409@qq.com
Mobile: 18021002903
QQ: 3008007409
Wechat: 18301782025
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